EPHB1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EPHB1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human EPHB1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of EPHB1 polyclonal antibody (Cat # PAB3018) in mouse brain tissue. EPHB1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of EPHB1 in human gastric cancer tissues.
(a) EPHB1 protein expressed in normal mucosa at the glandular compartment and in a decreasing gradient from the glandular compartment to the foveolar compartment.
(b) EPHB1 protein focally positively stained in well-differentiated gastric cancer cells.
(c) EPHB1 protein is focally positive in poorly differentiated gastric cancer cells.
(d) Loss of EPHB1 expression in gastric cancer cells. (Provided by Jian-dong Wang,Department of Pathology Nanjing Jinling Hospital/Nanjing University School of Medicine) -
Gene Info — EPHB1
Entrez GeneID
2047Protein Accession#
P54762Gene Name
EPHB1
Gene Alias
ELK, EPHT2, FLJ37986, Hek6, NET
Gene Description
EPH receptor B1
Omim ID
600600Gene Ontology
HyperlinkGene Summary
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq
Other Designations
eph tyrosine kinase 2|ephrin receptor EphB1|soluble EPHB1 variant 1
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Interactome
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Publication Reference
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Downstream mediators of Ten-m3 signalling in the developing visual pathway.
Glendining KA, Liu SC, Nguyen M, Dharmaratne N, Nagarajah R, Iglesias MA, Sawatari A, Leamey CA.
BMC Neuroscience 2017 Dec; 18(1):78.
Application:WB-Ti, Mouse, Mouse brain.
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EphB1 is underexpressed in poorly differentiated colorectal cancers.
Sheng Z, Wang J, Dong Y, Ma H, Zhou H, Sugimura H, Lu G, Zhou X.
Pathobiology 2008 Oct; 75(5):274.
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Loss of expression of EphB1 protein in gastric carcinoma associated with invasion and metastasis.
Wang JD, Dong YC, Sheng Z, Ma HH, Li GL, Wang XL, Lu GM, Sugimura H, Jin J, Zhou XJ.
Oncology 2008 Apr; 73(3-4):238.
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Downstream mediators of Ten-m3 signalling in the developing visual pathway.
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