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Last updated: 2017/12/17

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P4HB polyclonal antibody

  • Catalog # : PAB30179
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial synthetic peptide of human P4HB.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to residues within 100-150 of human P4HB.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 60
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Lyophilized
  • Purification:
  • Immunoaffinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.05-0.2 ug/mL)
    Flow Cytometry (1-3 ug/106 cells)
    Western Blot (0.5-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C or lower.
    Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
    The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: K562, Lane 2: HepG2, Lane 3: A431, Lane 4: Hela, Lane 5: HEK293, Lane 6: Ramos cell lysates, Lane 7: mouse brain, Lane 8: rat brain tissue lysates with P4HB polyclonal antibody (Cat # PAB30179).
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HepG2 cells with P4HB polyclonal antibody (Cat # PAB30179) (shaded histogram). Open histogram: isotype control.
  • Application Image
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5034
  • Gene Name:
  • P4HB
  • Gene Alias:
  • DSI,ERBA2L,GIT,P4Hbeta,PDI,PDIA1,PHDB,PO4DB,PO4HB,PROHB
  • Gene Description:
  • prolyl 4-hydroxylase, beta polypeptide
  • Gene Summary:
  • This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq
  • Other Designations:
  • collagen prolyl 4-hydroxylase beta,glutathione-insulin transhydrogenase,procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide,prolyl 4-hydroxylase, beta subunit,protein disulfide isomerase family A, member 1,protein d
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