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NES polyclonal antibody

  • Catalog # : PAB30177
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial synthetic peptide of human NES.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to residues within 1280-1330 of human NES.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 220
  • Reactivity:
  • Human
  • Form:
  • Lyophilized
  • Purification:
  • Immunoaffinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.05-0.2 ug/mL)
    Western Blot (0.5-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C or lower.
    Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
    The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of SH-SY5Y cell lysates with Lane 1: NES polyclonal antibody (Cat # PAB30177), Lane 2: the same antibody pre-incubated with blocking peptide.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • NES
  • Gene Alias:
  • FLJ21841,Nbla00170
  • Gene Description:
  • nestin
  • Gene Summary:
  • Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000032198
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