Rabbit polyclonal antibody raised against partial synthetic peptide of human VIM.
A synthetic peptide (conjugated with KLH) corresponding to residues within 400-500 of human VIM.
Theoretical MW (kDa):
ELISA (0.05-0.2 ug/mL) Flow Cytometry (1-3 ug/106 cells) Immunohistochemistry (5-10 ug/mL) Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02% sodium azide).
Store at -20°C or lower. Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL. The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of HeLa and HEK293 cell lysates using VIM polyclonal antibody (Cat # PAB30175). Lane 1: HeLa cell lysate stained with Cat # PAB30175. Lane 2: HEK293 cell lysate stained with Cat # PAB30175. Lane 3: HeLa cell lysate stained with Cat # PAB30175 pre-incubated with immunizing peptide. Lane 4: HEK293 cell lysate stained with Cat # PAB30175 pre-incubated with immunizing peptide. Additional band size 30 kDa (Possible VIM fragment).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human spleen tissue with VIM polyclonal antibody (Cat # PAB30175) (left) and purified rabbit IgG (whole molecule) control (right).
Flow cytometric analysis of Ramos cells with VIM polyclonal antibody (Cat # PAB30175) (shaded histogram). Open histogram: isotype control.
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract