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YWHAZ (phospho S58) polyclonal antibody

  • Catalog # : PAB30172
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial synthetic phosphopeptide of human YWHAZ.
  • Immunogen:
  • A synthetic phosphopeptide corresponding to residues surrounding S58 of human YWHAZ.
  • Sequence:
  • R-S-Sp-W-R
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunofluorescence (1:100-1:200)
    Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 1.0 mg/mL PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C for short term use. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extract from Lane 1: untreated NIH/3T3 cell and Lane 2: NIH/3T3 cell treated with TNF-alpha (20 ng/mL, 5 min) using YWHAZ (phospho S58) polyclonal antibody (Cat # PAB30172).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast carcinoma tissue with YWHAZ (phospho S58) polyclonal antibody (Cat # PAB30172) (left) or the same antibody pre-incubated with blocking peptide (right).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of methanol-fixed HeLa cells showing cytoplasmic and centrosomal staining with YWHAZ (phospho S58) polyclonal antibody (Cat # PAB30172).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7534
  • Gene Name:
  • Gene Alias:
  • KCIP-1,MGC111427,MGC126532,MGC138156
  • Gene Description:
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
  • Gene Summary:
  • This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • 14-3-3 protein/cytosolic phospholipase A2,14-3-3 zeta,OTTHUMP00000165851,OTTHUMP00000165852,OTTHUMP00000165854,OTTHUMP00000165858,OTTHUMP00000165859,OTTHUMP00000165860,phospholipase A2,protein kinase C inhibitor protein-1,tyrosine 3/tryptophan 5 -monooxyg
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