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PKC alpha (T638)/PKC beta II (T641) polyclonal antibody

  • Catalog # : PAB30171
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial synthetic phosphopeptide of human PKC alpha/PKC beta II.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T638 of human PKC alpha and T641 of human PKC beta II.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody detects endogenous levels of PKC alpha only when phosphorylated at threonine 638 and PKC beta II only when phosphorylated at threonine 641.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.05-0.2 ug/mL)
    Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.5 mg/mL PBS, pH 7.4 (30% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C is stable for 2-3 weeks. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of lysates from Hela cells, untreated (Lane 1, 3, 5) or TPA-treated (Lane 2, 4, 6), with PKC alpha (T638)/PKC beta II (T641) polyclonal antibody (Cat # PAB30171).
    Lane 1, 2: PKC alpha (T638)/PKC beta II (T641) polyclonal antibody (Cat # PAB30171)
    Lane 3, 4: the same antibody pre-incubated with nonphospho-peptide
    Lane 5, 6: the same antibody pre-incubated with phospho-peptide.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of NIH/3T3 cell lysates with Lane 1: PKC alpha (T638)/PKC beta II (T641) polyclonal antibody (Cat # PAB30171). Lane 2: the same antibody pre-incubated with nonphospho-peptide, Lane 3: the same antibody pre-incubated with phospho-peptide.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5578
  • Protein Accession#:
  • P17252; P05771
  • Gene Name:
  • PRKCA
  • Gene Alias:
  • AAG6,MGC129900,MGC129901,PKC-alpha,PKCA,PRKACA
  • Gene Description:
  • protein kinase C, alpha
  • Gene Summary:
  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq
  • Other Designations:
  • aging-associated gene 6,protein kinase C alpha type
  • Gene Information
  • Entrez GeneID:
  • 5579
  • Protein Accession#:
  • P17252; P05771
  • Gene Name:
  • PRKCB
  • Gene Alias:
  • MGC41878,PKC-beta,PKCB,PRKCB1,PRKCB2
  • Gene Description:
  • protein kinase C, beta
  • Gene Summary:
  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • protein kinase C, beta 1 polypeptide
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