Rabbit polyclonal antibody raised against partial synthetic phosphopeptide of human MAPT.
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S409 of human MAPT.
ELISA (0.05-0.2 ug/mL) Western Blot (0.5-2 ug/mL) The optimal working dilution should be determined by the end user.
Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02% sodium azide).
Store at -20°C or lower. Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL. The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of phosphorylated recombinant human MAPT protein, stimulated by GSK3beta, with Lane 1: MAPT (phospho S409) polyclonal antibody (Cat # PAB30170). Lane 2: the same antibody pre-incubated with nonphospho-peptide, Lane 3: the same antibody pre-incubated with phospho-peptide.
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq
G protein beta1/gamma2 subunit-interacting factor 1,microtubule-associated protein tau, isoform 4