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RPS6 (phospho S235) polyclonal antibody

  • Catalog # : PAB30165
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial synthetic phosphopeptide of human RPS6.
  • Immunogen:
  • A synthetic phosphopeptide corresponding to residues surrounding S235 of human RPS6.
  • Sequence:
  • R-L-Sp-S-L
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 32
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunofluorescence (1:100-1:200)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 1.0 mg/mL PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C for short term use. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extract from Lane 1: untreated 293 cell and Lane 2: 293 cell treated with serum (10%, 15 min) using RPS6 (phospho S235) polyclonal antibody (Cat # PAB30165).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of methanol-fixed HeLa cells with RPS6 (phospho S235) polyclonal antibody (Cat # PAB30165).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6194
  • Gene Name:
  • RPS6
  • Gene Alias:
  • -
  • Gene Description:
  • ribosomal protein S6
  • Gene Summary:
  • Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
  • Other Designations:
  • 40S ribosomal protein S6,OTTHUMP00000021120,phosphoprotein NP33
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