GPAA1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of human GPAA1.
Immunogen
A synthetic peptide corresponding to C-terminus of human GPAA1.
Sequence
LGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGL
Host
Rabbit
Theoretical MW (kDa)
67
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunofluorescence (1:100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-8 ug/mL)
Western Blot (0.2-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (2% sucrose, 0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HepG2 cell lysate with GPAA1 polyclonal antibody (Cat # PAB30107) at 0.2-1 ug/mL working concentration.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with GPAA1 polyclonal antibody (Cat # PAB30107) at 4-8 ug/mL working concentration.Immunofluorescence
Immunofluorescent staining of human liver (A) and human lung (B) with GPAA1 polyclonal antibody (Cat # PAB30107) at 1:100 dilution. -
Gene Info — GPAA1
Entrez GeneID
8733GeneBank Accession#
NM_003801Protein Accession#
NP_003792;O43292Gene Name
GPAA1
Gene Alias
GAA1, hGAA1
Gene Description
glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
Omim ID
603048Gene Ontology
HyperlinkGene Summary
Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains. [provided by RefSeq
Other Designations
GPAA1P anchor attachment protein 1 homolog|GPI transamidase subunit|anchor attachment protein 1 (Gaa1p, yeast) homolog|glycophosphatidylinositol anchor attachment 1|glycosylphosphatidylinositol anchor attachment protein 1
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Interactome
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Pathway
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