GOT2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western Blot analysis of HepG2 cell lysate with GOT2 polyclonal antibody (Cat # PAB30106) at 1.25 ug/mL working concentration.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver with GOT2 polyclonal antibody (Cat # PAB30106) at 4-8 ug/mL working concentration.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of human GOT2.
Immunogen
A synthetic peptide corresponding to C-terminus of human GOT2.
Sequence
AILNTPDLRKQWLQEVKVMADRIIGMRTQLVSNLKKEGSTHNWQHITDQI
Host
Rabbit
Theoretical MW (kDa)
45
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-8 ug/mL)
Western Blot (1.25 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (2% sucrose, 0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HepG2 cell lysate with GOT2 polyclonal antibody (Cat # PAB30106) at 1.25 ug/mL working concentration.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver with GOT2 polyclonal antibody (Cat # PAB30106) at 4-8 ug/mL working concentration. -
Gene Info — GOT2
Entrez GeneID
2806GeneBank Accession#
NM_002080Protein Accession#
NP_002071;P00505Gene Name
GOT2
Gene Alias
FLJ40994, KAT4, KATIV, mitAAT
Gene Description
glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
Omim ID
138150Gene Ontology
HyperlinkGene Summary
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq
Other Designations
aspartate aminotransferase 2|kynurenine aminotransferase IV
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Interactome
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Pathway
- Alanine
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Carbon fixation in photosynthetic organisms
- Cysteine and methionine metabolism
- Isoquinoline alkaloid biosynthesis
- Metabolic pathways
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