EPHA4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EPHA4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 40-70 at N-terminus of human EPHA4.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Flow Cytometry(1:10-1:50)
Immunofluorescence (1:100)
Immunohistochemistry (1:50-1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EPHA4 polyclonal antibody (Cat # PAB3007) in HeLa cell lysate. EPHA4 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Western Blot (Transfected lysate)
Western blot analysis of EPHA4 (arrow) using EPHA4 polyclonal antibody (Cat # PAB3007).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA4 polyclonal antibody (Cat # PAB3007) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.Immunofluorescence
Methanol/Acetone fixed human stem cell is used in IF to detect EPHA4 (blue) and endothelial Lectin (red) . Data kindly provided by Dr. Weis from Cheresh Lab, UCSD.Flow Cytometry
Flow cytometric analysis of CEM cells using EPHA4 polyclonal antibody (Cat # PAB3007)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — EPHA4
Entrez GeneID
2043Protein Accession#
P54764Gene Name
EPHA4
Gene Alias
HEK8, SEK, TYRO1
Gene Description
EPH receptor A4
Omim ID
602188Gene Ontology
HyperlinkGene Summary
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq
Other Designations
OTTHUMP00000164185|TYRO1 protein tyrosine kinase|ephrin receptor EphA4|ephrin type-A receptor 4|receptor protein-tyrosine kinase HEK8|tyrosine-protein kinase receptor SEK
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Interactome
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Pathway
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Disease
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Publication Reference
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Eph/Ephrin Profiling in Human Breast Cancer Reveals Significant Associations between Expression Level and Clinical Outcome.
Brantley-Sieders DM, Jiang A, Sarma K, Badu-Nkansah A, Walter DL, Shyr Y, Chen J.
PLoS One 2011 Sep; 6(9):e24426.
Application:IHC-P, Human, Mouse, Human ductal carcinoma, Mouse kidney tissues.
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Roles of Eph receptors and ephrins in segmental patterning.
Xu Q, Mellitzer G, Wilkinson DG.
Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 2000 Jul; 355(1399):993.
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Eph receptors and ephrins: effectors of morphogenesis.
Holder N, Klein R.
Development 1999 May; 126(10):2033.
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Eph/Ephrin Profiling in Human Breast Cancer Reveals Significant Associations between Expression Level and Clinical Outcome.
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