Western blot analysis of EPHA3 polyclonal antibody (Cat # PAB3006) in CHO cell lysate. EPHA3 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot (Transfected lysate)
Western blot analysis of EPHA3 (arrow) using EPHA3 polyclonal antibody (Cat # PAB3006). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA3 gene (Lane 2).
Formalin-fixed and paraffin-embedded human placenta tissue reacted with EPHA3 polyclonal antibody (Cat # PAB3006), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq