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Last updated: 2016/10/23
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EPHA2 polyclonal antibody

  • Catalog # : PAB3003
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of EPHA2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EPHA2.
  • Host:
  • Rabbit
  • Reactivity:
  • Hamster, Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:50-100 Flow cytometry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The EPHA2 polyclonal antibody (Cat # PAB3003) is used in Western blot to detect EPHA2 in CHO cell lysate (Lane 1) and NIH/3T3 cell lysate (Lane 2) .
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA2 polyclonal antibody (Cat # PAB3003) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of NCI-H292 cells using EPHA2 polyclonal antibody (Cat # PAB3003)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1969
  • Gene Name:
  • EPHA2
  • Gene Alias:
  • ECK
  • Gene Description:
  • EPH receptor A2
  • Gene Summary:
  • This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. [provided by RefSeq
  • Other Designations:
  • ephrin receptor EphA2,epithelial cell receptor protein tyrosine kinase,protein tyrosine kinase,receptor protein tyrosine kinase regulated by p53 and E2F-1,soluble EPHA2 variant 1
  • Gene Pathway
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