ASS1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of human ASS1.
Immunogen
A synthetic peptide corresponding to N-terminus of human ASS1.
Sequence
YSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVF
Host
Rabbit
Theoretical MW (kDa)
45
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-8 ug/mL)
Western Blot (2.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (2% sucrose, 0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HepG2 cell lysate with ASS1 polyclonal antibody (Cat # PAB29973) at 2.5 ug/mL working concentration.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with ASS1 polyclonal antibody (Cat # PAB29973) at 4-8 ug/mL working concentration. -
Gene Info — ASS1
Entrez GeneID
445GeneBank Accession#
NM_054012Protein Accession#
NP_446464;P00966Gene Name
ASS1
Gene Alias
ASS, CTLN1
Gene Description
argininosuccinate synthetase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000022362|OTTHUMP00000022363|OTTHUMP00000022364|citrulline--aspartate ligase
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Interactome
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Disease
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