CENPN polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of human CENPN.
Immunogen
A synthetic peptide corresponding to internal region of human CENPN.
Sequence
SFKKILQRALKNVTVSFRETEENAVWIRIAWGTQYTKPNQYKPTYVVYYS
Host
Rabbit
Theoretical MW (kDa)
41
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunofluorescence (1:250)
Western Blot (0.2-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (2% sucrose, 0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate with CENPN polyclonal antibody (Cat # PAB29940) at 0.2-1 ug/mL working concentration.Immunofluorescence
Immunofluorescent staining of HeLa (A), Huh7 (B) and MCF7 (C) cells with CENPN polyclonal antibody (Cat # PAB29940) at 1:250 dilution. -
Gene Info — CENPN
Entrez GeneID
55839GeneBank Accession#
NM_001100625Protein Accession#
NP_001094095;Q96H22Gene Name
CENPN
Gene Alias
BM039, C16orf60, CENP-N, FLJ13607, FLJ22660
Gene Description
centromere protein N
Omim ID
611509Gene Ontology
HyperlinkGene Summary
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPN is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM
Other Designations
OTTHUMP00000174973|OTTHUMP00000174974
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