CUL4A polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of CUL4A (arrow) using rabbit CUL4A polyclonal antibody (Cat # PAB2988). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CUL4A gene (Lane 2) (Origene Technologies).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CUL4A.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CUL4A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of CUL4A (arrow) using rabbit CUL4A polyclonal antibody (Cat # PAB2988). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CUL4A gene (Lane 2) (Origene Technologies). -
Gene Info — CUL4A
Entrez GeneID
8451Protein Accession#
NP_003580;Q13619Gene Name
CUL4A
Gene Alias
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Gene Description
cullin 4A
Omim ID
603137Gene Ontology
HyperlinkOther Designations
OTTHUMP00000018753|OTTHUMP00000018754
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Interactome
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Pathway
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Publication Reference
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WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase.
Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y.
Genes & Development 2008 Apr; 22(7):866.
Application：WB-Tr, Human, U2OS cells.
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VprBP targets Merlin to the Roc1-Cul4A-DDB1 E3 ligase complex for degradation.
Huang J, Chen J.
Oncogene 2008 Jul; 27(29):4056.
Application：WB-Tr, Human, HEK 293T cells.
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The HIV1 protein Vpr acts to promote G2 cell cycle arrest by engaging a DDB1 and Cullin4A-containing ubiquitin ligase complex using VprBP/DCAF1 as an adaptor.
Wen X, Duus KM, Friedrich TD, de Noronha CM.
The Journal of Biological Chemistry 2007 Sep; 282(37):27046.
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WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase.
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