HNRNPL polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Jurkat cell lysate with HNRNPL polyclonal antibody (Cat # PAB29878).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human heart and (B) human liver tissues with HNRNPL polyclonal antibody (Cat # PAB29878).
Immunofluorescence
Immunofluorescent staining of MCF-7 cell with HNRNPL polyclonal antibody (Cat # PAB29878) at 1:200 dilution.
Immunoprecipitation
Immunoprecipitation analysis of Lane 1: 5% Input, Lane 2: 5% Sup, Lane 3: Normal IgG, and Lane 4: HNRNPL ppt. K-562 sample with HNRNPL polyclonal antibody (Cat # PAB29878) at 1:4000 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial synthetic protein of human HNRNPL.
Immunogen
A synthetic peptide corresponding to amino acids 82-131 of human HNRNPL.
Sequence
AAGGGGGGENYDDPHKTPASPVVHIRGLIDGVVEADLVEALQEFGPISYV
Host
Rabbit
Theoretical MW (kDa)
65
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:200)
Immunohistochemistry (1:250)
Immunoprecipitation
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (2% sucrose, 0.09% sodium azide).
Storage Instruction
Store at 4°C for up to 1 week. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat cell lysate with HNRNPL polyclonal antibody (Cat # PAB29878).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human heart and (B) human liver tissues with HNRNPL polyclonal antibody (Cat # PAB29878).Immunofluorescence
Immunofluorescent staining of MCF-7 cell with HNRNPL polyclonal antibody (Cat # PAB29878) at 1:200 dilution.Immunoprecipitation
Immunoprecipitation analysis of Lane 1: 5% Input, Lane 2: 5% Sup, Lane 3: Normal IgG, and Lane 4: HNRNPL ppt. K-562 sample with HNRNPL polyclonal antibody (Cat # PAB29878) at 1:4000 dilution. -
Gene Info — HNRNPL
Entrez GeneID
3191Protein Accession#
P14866Gene Name
HNRNPL
Gene Alias
FLJ35509, HNRPL, P/OKcl.14, hnRNP-L
Gene Description
heterogeneous nuclear ribonucleoprotein L
Omim ID
603083Gene Ontology
HyperlinkGene Summary
Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
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