RASGRF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of MDA-MB-231 cell lysate (35 ug/lane) with RASGRF1 polyclonal antibody (Cat # PAB2980).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with RASGRF1 polyclonal antibody (Cat # PAB2980) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RASGRF1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S929 of human RASGRF1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MDA-MB-231 cell lysate (35 ug/lane) with RASGRF1 polyclonal antibody (Cat # PAB2980).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with RASGRF1 polyclonal antibody (Cat # PAB2980) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — RASGRF1
Entrez GeneID
5923Protein Accession#
NP_002882;Q13972Gene Name
RASGRF1
Gene Alias
CDC25, CDC25L, GNRP, GRF1, GRF55, H-GRF55, PP13187
Gene Description
Ras protein-specific guanine nucleotide-releasing factor 1
Omim ID
606600Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
Ras-specific guanine nucleotide-releasing factor, CDC25 homolog|Ras-specific nucleotide exchange factor CDC25|guanine nucleotide exchange factor|guanine nucleotide-releasing factor, 55 kD
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Interactome
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Pathway
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Disease
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Publication Reference
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Zoledronate-induced S phase arrest and apoptosis accompanied by DNA damage and activation of the ATM/Chk1/cdc25 pathway in human osteosarcoma cells.
Iguchi T, Miyakawa Y, Saito K, Nakabayashi C, Nakanishi M, Saya H, Ikeda Y, Kizaki M.
International Journal of Oncology 2007 Aug; 31(2):285.
Application：WB-Ce, Human, HOS cells.
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Timing and sequence requirements defined for embryonic maintenance of imprinted DNA methylation at Rasgrf1.
Holmes R, Chang Y, Soloway PD.
Molecular Biology of the Cell 2006 Dec; 26(24):9564.
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Zoledronate-induced S phase arrest and apoptosis accompanied by DNA damage and activation of the ATM/Chk1/cdc25 pathway in human osteosarcoma cells.
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