HARS polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HARS.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human HARS.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HARS polyclonal antibody (Cat # PAB2968) in K-562 cell line lysates (35 ug/lane).HARS (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with HARS polyclonal antibody (Cat # PAB2968), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — HARS
Entrez GeneID
3035Protein Accession#
NP_002100;P12081Gene Name
HARS
Gene Alias
FLJ20491, HRS
Gene Description
histidyl-tRNA synthetase
Omim ID
142810Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. [provided by RefSeq
Other Designations
HisRS|histidine tRNA ligase 1, cytoplasmic|histidine translase|histidine-tRNA ligase
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Interactome
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Pathway
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Publication Reference
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Novel conformation of histidyl-transfer RNA synthetase in the lung: the target tissue in Jo-1 autoantibody-associated myositis.
Levine SM, Raben N, Xie D, Askin FB, Tuder R, Mullins M, Rosen A, Casciola-Rosen LA.
Arthritis and Rheumatism 2007 Aug; 56(8):2729.
Application:IHC-P, WB, Human, Normal human tissues.
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TSG101 interaction with HRS mediates endosomal trafficking and receptor down-regulation.
Lu Q, Hope LW, Brasch M, Reinhard C, Cohen SN.
PNAS 2003 Jun; 100(13):7626.
Application:WB, Human, HeLa cells.
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Novel conformation of histidyl-transfer RNA synthetase in the lung: the target tissue in Jo-1 autoantibody-associated myositis.
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