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STK4/STK3 (phospho T183) polyclonal antibody

  • Catalog # : PAB29650
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human STK4/STK3.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T183 of human STK4/STK3.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 60
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • STK4/STK3 (phospho T183) polyclonal antibody detects endogenous levels of human STK4/STK3 only when phosphorylated at threonine 183.
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Untreated 293 cell lysates, Lane 2: Synthesized peptide treated 293 cell lysates reacted with STK4/STK3 (phospho T183) polyclonal antibody (Cat # PAB29650) at 1:500-1:3000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded human brain tissue reacted with STK4/STK3 (phospho T183) polyclonal antibody (Cat # PAB29650) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6789
  • Protein Accession#:
  • Q13043;Q13188
  • Gene Name:
  • STK4
  • Gene Alias:
  • DKFZp686A2068,KRS2,MST1,YSK3
  • Gene Description:
  • serine/threonine kinase 4
  • Gene Summary:
  • The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000043418,dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2)),kinase responsive to stress 2,mammalian sterile 20-like 1,yeast Ste20-like
  • Gene Information
  • Entrez GeneID:
  • 6788
  • Protein Accession#:
  • Q13043;Q13188
  • Gene Name:
  • STK3
  • Gene Alias:
  • FLJ90748,KRS1,MST2
  • Gene Description:
  • serine/threonine kinase 3 (STE20 homolog, yeast)
  • Gene Summary:
  • Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM
  • Other Designations:
  • serine/threonine kinase 3 (Ste20, yeast homolog)
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