EPRS polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of EPRS polyclonal antibody (Cat # PAB2965) in Jurkat cell line lysates (35 ug/lane). EPRS (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EPRS polyclonal antibody (Cat # PAB2965) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EPRS.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EPRS.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EPRS polyclonal antibody (Cat # PAB2965) in Jurkat cell line lysates (35 ug/lane). EPRS (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EPRS polyclonal antibody (Cat # PAB2965) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — EPRS
Entrez GeneID
2058Protein Accession#
NP_004437;P07814Gene Name
EPRS
Gene Alias
DKFZp313B047, EARS, GLUPRORS, PARS, PIG32, QARS, QPRS
Gene Description
glutamyl-prolyl-tRNA synthetase
Omim ID
138295Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000035562|bifunctional aminoacyl-tRNA synthetase|glutamate tRNA ligase|glutaminyl-tRNA synthetase|glutamyl-prolyl tRNA synthetase|proliferation-inducing protein 32|proline tRNA ligase|proline-tRNA ligase|prolyl-tRNA synthetase
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Interactome
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Pathway
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Disease
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Publication Reference
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WHEP domains direct noncanonical function of glutamyl-Prolyl tRNA synthetase in translational control of gene expression.
Jia J, Arif A, Ray PS, Fox PL.
Molecular Cell 2008 Mar; 29(6):679.
Application：IP, WB, Recombinant protein.
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A probability-based approach for high-throughput protein phosphorylation analysis and site localization.
Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP.
Nature Biotechnology 2006 Oct; 24(10):1285.
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A novel human tRNA-dihydrouridine synthase involved in pulmonary carcinogenesis.
Kato T, Daigo Y, Hayama S, Ishikawa N, Yamabuki T, Ito T, Miyamoto M, Kondo S, Nakamura Y.
Cancer Research 2005 Jul; 65(13):5638.
Application：IF, IHC-P, IP, WB, Human, Human lung cancer, A549, LC319, PC-14, SAEC, SBC-5, SK-MES-1 cells.
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WHEP domains direct noncanonical function of glutamyl-Prolyl tRNA synthetase in translational control of gene expression.
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