RPS6KA4 (phospho T568) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated 293 cell lysates, Lane 2: Synthesized peptide treated 293 cell lysates reacted with RPS6KA4 (phospho T568) polyclonal antibody (Cat # PAB29649) at 1:500-1:3000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human brain tissue reacted with RPS6KA4 (phospho T568) polyclonal antibody (Cat # PAB29649) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RPS6KA4.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T568 of human RPS6KA4.
Host
Rabbit
Theoretical MW (kDa)
95
Reactivity
Human, Mouse
Specificity
RPS6KA4 (phospho T568) polyclonal antibody detects endogenous levels of human RPS6KA4 only when phosphorylated at threonine 568.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated 293 cell lysates, Lane 2: Synthesized peptide treated 293 cell lysates reacted with RPS6KA4 (phospho T568) polyclonal antibody (Cat # PAB29649) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human brain tissue reacted with RPS6KA4 (phospho T568) polyclonal antibody (Cat # PAB29649) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide. -
Gene Info — RPS6KA4
Entrez GeneID
8986Protein Accession#
O75676Gene Name
RPS6KA4
Gene Alias
MSK2, RSK-B
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 4
Omim ID
603606Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq
Other Designations
mitogen- and stress-activated protein kinase 2|ribosomal protein S6 kinase alpha 4|ribosomal protein S6 kinase, 90kD, polypeptide 4|ribosomal protein kinase B
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Interactome
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Pathway
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