Western blot analysis of Lane 1: Untreated HeLa cell lysates, Lane 2: Synthesized peptide treated HeLa cell lysates reacted with MDM4 (phospho S367) polyclonal antibody (Cat # PAB29648) at 1:500-1:3000 dilution.
Immunohistochemical staining of paraffin-embedded human colon carcinoma tissue reacted with MDM4 (phospho S367) polyclonal antibody (Cat # PAB29648) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Two transcript variants, one protein-coding and the other likely not to be protein-coding, have been found for this gene. [provided by RefSeq
MDM4-related protein 1,Mdm4, transformed 3T3 cell double minute 4, p53 binding protein,double minute 4, human homolog of; p53-binding protein,mouse double minute 4 homolog,p53-binding protein