MDM4 (phospho S367) polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human MDM4.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S367 of human MDM4.
Host
Rabbit
Theoretical MW (kDa)
80
Reactivity
Human, Mouse, Rat
Specificity
MDM4 (phospho S367) polyclonal antibody detects endogenous levels of human MDM4 only when phosphorylated at serine 367.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated HeLa cell lysates, Lane 2: Synthesized peptide treated HeLa cell lysates reacted with MDM4 (phospho S367) polyclonal antibody (Cat # PAB29648) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human colon carcinoma tissue reacted with MDM4 (phospho S367) polyclonal antibody (Cat # PAB29648) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide. -
Gene Info — MDM4
Entrez GeneID
4194Protein Accession#
O15151Gene Name
MDM4
Gene Alias
DKFZp781B1423, HDMX, MDMX, MGC132766, MRP1
Gene Description
Mdm4 p53 binding protein homolog (mouse)
Omim ID
602704Gene Ontology
HyperlinkGene Summary
The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Two transcript variants, one protein-coding and the other likely not to be protein-coding, have been found for this gene. [provided by RefSeq
Other Designations
MDM4-related protein 1|Mdm4, transformed 3T3 cell double minute 4, p53 binding protein|double minute 4, human homolog of; p53-binding protein|mouse double minute 4 homolog|p53-binding protein
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