Western blot analysis of Lane 1: Untreated 293 cell lysates, Lane 2: Synthesized peptide treated 293 cell lysates reacted with ATRIP (phospho S224) polyclonal antibody (Cat # PAB29644) at 1:500-1:3000 dilution.
Immunohistochemical staining of paraffin-embedded human colon carcinoma tissue reacted with ATRIP (phospho S224) polyclonal antibody (Cat # PAB29644) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
The product of this gene is an essential component of the DNA damage checkpoint, and binds to single-stranded DNA coated with replication protein A that accumulates at sites of DNA damage. The encoded protein interacts with the ataxia telangiectasia and Rad3 related protein, a checkpoint kinase, resulting in accumulation of the kinase at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq