ALK (phospho Y1604) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated HepG2 cell lysates, Lane 2: Synthesized peptide treated HepG2 cell lysates reacted with ALK (phospho Y1604) polyclonal antibody (Cat # PAB29642) at 1:500-1:3000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human brain tissue reacted with ALK (phospho Y1604) polyclonal antibody (Cat # PAB29642) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
Immunofluorescence
Immunofluorescent staining of HeLa cells reacted with ALK (phospho Y1604) polyclonal antibody (Cat # PAB29642) at 1:100-1:500 dilution. The picture on the right is treated with the synthesized peptide.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human ALK.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y1604 of human ALK.
Host
Rabbit
Theoretical MW (kDa)
176
Reactivity
Human
Specificity
ALK (phospho Y1604) polyclonal antibody detects endogenous levels of human ALK only when phosphorylated at tyrosine 1604.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:100-1:500)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated HepG2 cell lysates, Lane 2: Synthesized peptide treated HepG2 cell lysates reacted with ALK (phospho Y1604) polyclonal antibody (Cat # PAB29642) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human brain tissue reacted with ALK (phospho Y1604) polyclonal antibody (Cat # PAB29642) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.Immunofluorescence
Immunofluorescent staining of HeLa cells reacted with ALK (phospho Y1604) polyclonal antibody (Cat # PAB29642) at 1:100-1:500 dilution. The picture on the right is treated with the synthesized peptide. -
Gene Info — ALK
Entrez GeneID
238Protein Accession#
Q9UM73Gene Name
ALK
Gene Alias
CD246, Ki-1, TFG/ALK
Gene Description
anaplastic lymphoma receptor tyrosine kinase
Omim ID
105590Gene Ontology
HyperlinkGene Summary
The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. [provided by RefSeq
Other Designations
ALK tyrosine kinase receptor|CD246 antigen|anaplastic lymphoma kinase (Ki-1)|anaplastic lymphoma kinase Ki-1
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Interactome
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Disease
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