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RIPK2 (phospho S176) polyclonal antibody

  • Catalog # : PAB29639
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RIPK2.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S176 of human RIPK2.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 70
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • RIPK2 (phospho S176) polyclonal antibody detects endogenous levels of human RIPK2 only when phosphorylated at serine 176.
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Untreated 293 cell lysates, Lane 2: Synthesized peptide treated 293 cell lysates reacted with RIPK2 (phospho S176) polyclonal antibody (Cat # PAB29639) at 1:500-1:3000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded human brain tissue reacted with RIPK2 (phospho S176) polyclonal antibody (Cat # PAB29639) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8767
  • Gene Name:
  • RIPK2
  • Gene Alias:
  • CARD3,CARDIAK,CCK,GIG30,RICK,RIP2
  • Gene Description:
  • receptor-interacting serine-threonine kinase 2
  • Gene Summary:
  • This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq
  • Other Designations:
  • CARD-carrying kinase,CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase,growth-inhibiting gene 30,receptor interacting protein 2,receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP
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