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AKT1/AKT3 (phospho Y437/Y434) polyclonal antibody

  • Catalog # : PAB29638
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human AKT1/AKT3.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y437/Y434 of human AKT1/AKT3.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 56
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • AKT1/AKT3 (phospho Y437/Y434) polyclonal antibody detects endogenous levels of human AKT1/AKT3 only when phosphorylated at tyrosine 437 and tyrosine 434.
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Untreated K562 cell lysates, Lane 2: Synthesized peptide treated K562 cell lysates reacted with AKT1/AKT3 (phospho Y437/Y434) polyclonal antibody (Cat # PAB29638) at 1:500-1:3000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded human brain tissue reacted with AKT1/AKT3 (phospho Y437/Y434) polyclonal antibody (Cat # PAB29638) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 207
  • Protein Accession#:
  • P31749;Q9Y243
  • Gene Name:
  • AKT1
  • Gene Alias:
  • AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
  • Gene Description:
  • v-akt murine thymoma viral oncogene homolog 1
  • Gene Summary:
  • The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
  • Other Designations:
  • RAC-alpha serine/threonine-protein kinase,murine thymoma viral (v-akt) oncogene homolog-1,protein kinase B,rac protein kinase alpha
  • Gene Information
  • Protein Accession#:
  • P31749;Q9Y243
  • Gene Name:
  • AKT3
  • Gene Alias:
  • DKFZp434N0250,PKB-GAMMA,PKBG,PRKBG,RAC-PK-gamma,RAC-gamma,STK-2
  • Gene Description:
  • v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
  • Gene Summary:
  • The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000037911,OTTHUMP00000037912,RAC-gamma serine/threonine protein kinase,protein kinase B gamma,serine threonine protein kinase, Akt-3,v-akt murine thymoma viral oncogene homolog 3
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