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TOP2A (phospho S1106) polyclonal antibody

  • Catalog # : PAB29634
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human TOP2A.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S1106 of human TOP2A.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 174
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • TOP2A (phospho S1106) polyclonal antibody detects endogenous levels of human TOP2A only when phosphorylated at serine 1106.
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Untreated Jurkat cell lysates, Lane 2: Synthesized peptide treated Jurkat cell lysates reacted with TOP2A (phospho S1106) polyclonal antibody (Cat # PAB29634) at 1:500-1:3000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue reacted with TOP2A (phospho S1106) polyclonal antibody (Cat # PAB29634) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7153
  • Gene Name:
  • TOP2A
  • Gene Alias:
  • TOP2,TP2A
  • Gene Description:
  • topoisomerase (DNA) II alpha 170kDa
  • Gene Summary:
  • This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq
  • Other Designations:
  • DNA topoisomerase II, 170 kD,DNA topoisomerase II, alpha isozyme,topoisomerase (DNA) II alpha (170kD)
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