KCNJ11 (phospho T224) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated HeLa cell lysates, Lane 2: Synthesized peptide treated HeLa cell lysates reacted with KCNJ11 (phospho T224) polyclonal antibody (Cat # PAB29633) at 1:500-1:3000 dilution.
Immunofluorescence
Immunofluorescent staining of HUVEC cells reacted with KCNJ11 (phospho T224) polyclonal antibody (Cat # PAB29633) at 1:100-1:500 dilution. The picture on the right is treated with the synthesized peptide.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human KCNJ11.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T224 of human KCNJ11.
Host
Rabbit
Theoretical MW (kDa)
40
Reactivity
Human, Mouse
Specificity
KCNJ11 (phospho T224) polyclonal antibody detects endogenous levels of human KCNJ11 only when phosphorylated at threonine 224.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunofluorescence (1:100-1:500)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated HeLa cell lysates, Lane 2: Synthesized peptide treated HeLa cell lysates reacted with KCNJ11 (phospho T224) polyclonal antibody (Cat # PAB29633) at 1:500-1:3000 dilution.Immunofluorescence
Immunofluorescent staining of HUVEC cells reacted with KCNJ11 (phospho T224) polyclonal antibody (Cat # PAB29633) at 1:100-1:500 dilution. The picture on the right is treated with the synthesized peptide. -
Gene Info — KCNJ11
Entrez GeneID
3767Protein Accession#
Q14654Gene Name
KCNJ11
Gene Alias
BIR, HHF2, IKATP, KIR6.2, MGC133230, PHHI, TNDM3
Gene Description
potassium inwardly-rectifying channel, subfamily J, member 11
Gene Ontology
HyperlinkGene Summary
Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and is found associated with the sulfonylurea receptor SUR. Mutations in this gene are a cause of familial persistent hyperinsulinemic hypoglycemia of infancy (PHHI), an autosomal recessive disorder characterized by unregulated insulin secretion. Defects in this gene may also contribute to autosomal dominant non-insulin-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM). [provided by RefSeq
Other Designations
ATP-sensitive inward rectifier potassium channel 11|beta-cell inward rectifier subunit|inwardly rectifying potassium channel KIR6.2|potassium inwardly-rectifying channel J11
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