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Last updated: 2017/7/23

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RB1 (phospho T826) polyclonal antibody

  • Catalog # : PAB29632
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RB1.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T826 of human RB1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 110
  • Reactivity:
  • Human, Mouse, Rabbit
  • Specificity:
  • RB1 (phospho T826) polyclonal antibody detects endogenous level of RB1 only when phosphorylated at threonine 826.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunofluorescence (1:100-1:200)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: nocodazole treated (1 ug/mL, 16 hours) HepG2 cells, Lane 2: blocking peptide treated HepG2 cells with RB1 (phospho T826) polyclonal antibody (Cat# PAB29632) at 1:500-1:1000 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of COS7 cells by RB1 (phospho T826) polyclonal antibody (Cat# PAB29632) without synthesized peptide (A) or preincubated with synthesized peptide (B) at 1:100-1:200 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5925
  • Gene Name:
  • RB1
  • Gene Alias:
  • OSRC,RB,p105-Rb,pRb,pp110
  • Gene Description:
  • retinoblastoma 1
  • Gene Summary:
  • The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000018397,retinoblastoma suspectibility protein,retinoblastoma-associated protein
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