Western blot analysis of Lane 1: nocodazole treated (1 ug/mL, 16 hours) HepG2 cells, Lane 2: blocking peptide treated HepG2 cells with RB1 (phospho T826) polyclonal antibody (Cat# PAB29632) at 1:500-1:1000 dilution.
Immunofluorescent staining of COS7 cells by RB1 (phospho T826) polyclonal antibody (Cat# PAB29632) without synthesized peptide (A) or preincubated with synthesized peptide (B) at 1:100-1:200 dilution.
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
OTTHUMP00000018397,retinoblastoma suspectibility protein,retinoblastoma-associated protein