RB1 (phospho T826) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RB1.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T826 of human RB1.
Host
Rabbit
Theoretical MW (kDa)
110
Reactivity
Human, Mouse, Rabbit
Specificity
RB1 (phospho T826) polyclonal antibody detects endogenous level of RB1 only when phosphorylated at threonine 826.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100-1:200)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: nocodazole treated (1 ug/mL, 16 hours) HepG2 cells, Lane 2: blocking peptide treated HepG2 cells with RB1 (phospho T826) polyclonal antibody (Cat# PAB29632) at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of COS7 cells by RB1 (phospho T826) polyclonal antibody (Cat# PAB29632) without synthesized peptide (A) or preincubated with synthesized peptide (B) at 1:100-1:200 dilution. -
Gene Info — RB1
Entrez GeneID
5925Protein Accession#
P06400Gene Name
RB1
Gene Alias
OSRC, RB, p105-Rb, pRb, pp110
Gene Description
retinoblastoma 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018397|retinoblastoma suspectibility protein|retinoblastoma-associated protein
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Interactome
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Disease
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