Fadd (phospho S190) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: PMA treated Jurkat cells, Lane 2: antigen-specific peptide treated Jurkat cells with Fadd (phospho S190) polyclonal antibody (Cat# PAB29631) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by Fadd (phospho S190) polyclonal antibody (Cat# PAB29631) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of mouse Fadd.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S190 of mouse Fadd.
Host
Rabbit
Theoretical MW (kDa)
25
Reactivity
Human, Mouse
Specificity
Fadd (phospho S190) polyclonal antibody detects endogenous level of Fadd only when phosphorylated at serine 190.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: PMA treated Jurkat cells, Lane 2: antigen-specific peptide treated Jurkat cells with Fadd (phospho S190) polyclonal antibody (Cat# PAB29631) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by Fadd (phospho S190) polyclonal antibody (Cat# PAB29631) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — Fadd
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