APP (phospho T743/668) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated cos-7 cells, Lane 2: cos-7 cells with APP (phospho T743/668) polyclonal antibody (Cat# PAB29627) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by APP (phospho T743/668) polyclonal antibody (Cat# PAB29627) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescent staining of methanol-fixed Hela cells using APP (phospho T743/668) polyclonal antibody (Cat# PAB29627) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human APP.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T743/668 of human APP.
Host
Rabbit
Theoretical MW (kDa)
140
Reactivity
Human, Mouse, Rabbit
Specificity
APP (phospho T743/668) polyclonal antibody detects endogenous level of APP only when phosphorylated at threonine 743/668.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated cos-7 cells, Lane 2: cos-7 cells with APP (phospho T743/668) polyclonal antibody (Cat# PAB29627) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by APP (phospho T743/668) polyclonal antibody (Cat# PAB29627) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.Immunofluorescence
Immunofluorescent staining of methanol-fixed Hela cells using APP (phospho T743/668) polyclonal antibody (Cat# PAB29627) at 1:100-1:200 dilution. -
Gene Info — APP
Entrez GeneID
351Protein Accession#
P05067Gene Name
APP
Gene Alias
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN2
Gene Description
amyloid beta (A4) precursor protein
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq
Other Designations
A4 amyloid protein|amyloid beta A4 protein|amyloid-beta protein|beta-amyloid peptide|cerebral vascular amyloid peptide|peptidase nexin-II|protease nexin-II
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Interactome
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Disease
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