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HIST1H3D (Di-methyl-K27) polyclonal antibody

  • Catalog # : PAB29623
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic Di-methyl-peptide of human HIST1H3D.
  • Immunogen:
  • A synthetic Di-methyl-peptide (conjugated with KLH) corresponding to residues surrounding K27 of human HIST1H3D.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 17
  • Reactivity:
  • Human, Mouse, Rabbit
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: C6 cells, Lane 2: Hela cells with HIST1H3D (Di-methyl-K27) polyclonal antibody (Cat# PAB29623) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human breast cancer tissue with HIST1H3D (Di-methyl-K27) polyclonal antibody (Cat# PAB29623) under 1:50-1:100 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8351
  • Gene Name:
  • HIST1H3D
  • Gene Alias:
  • H3/b,H3FB
  • Gene Description:
  • histone cluster 1, H3d
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
  • Other Designations:
  • H3 histone family, member B,OTTHUMP00000016149,histone 1, H3d
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