HIST1H3D (Di-methyl-K27) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: C6 cells, Lane 2: Hela cells with HIST1H3D (Di-methyl-K27) polyclonal antibody (Cat# PAB29623) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue with HIST1H3D (Di-methyl-K27) polyclonal antibody (Cat# PAB29623) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic Di-methyl-peptide of human HIST1H3D.
Immunogen
A synthetic Di-methyl-peptide (conjugated with KLH) corresponding to residues surrounding K27 of human HIST1H3D.
Host
Rabbit
Theoretical MW (kDa)
17
Reactivity
Human, Mouse, Rabbit
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: C6 cells, Lane 2: Hela cells with HIST1H3D (Di-methyl-K27) polyclonal antibody (Cat# PAB29623) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue with HIST1H3D (Di-methyl-K27) polyclonal antibody (Cat# PAB29623) under 1:50-1:100 dilution. -
Gene Info — HIST1H3D
Entrez GeneID
8351Protein Accession#
P68431Gene Name
HIST1H3D
Gene Alias
H3/b, H3FB
Gene Description
histone cluster 1, H3d
Omim ID
602811Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H3 histone family, member B|OTTHUMP00000016149|histone 1, H3d
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Interactome
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Pathway
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