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PRKCQ (phospho S676) polyclonal antibody

  • Catalog # : PAB29619
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human PRKCQ.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S676 of human PRKCQ.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 80
  • Reactivity:
  • Human, Mouse, Rabbit
  • Specificity:
  • PRKCQ (phospho S676) polyclonal antibody detects endogenous level of PRKCQ only when phosphorylated at serine 676.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunofluorescence (1:100-1:200)
    Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: PC12 cells, Lane 2: PMA treated PC12 cells with PRKCQ (phospho S676) polyclonal antibody (Cat# PAB29619) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human lung cancer tissue by PRKCQ (phospho S676) polyclonal antibody (Cat# PAB29619) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of methanol-fixed Hela cells using PRKCQ (phospho S676) polyclonal antibody (Cat# PAB29619) at 1:100-1:200 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5588
  • Gene Name:
  • PRKCQ
  • Gene Alias:
  • MGC126514,MGC141919,PRKCT,nPKC-theta
  • Gene Description:
  • protein kinase C, theta
  • Gene Summary:
  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019053,OTTHUMP00000043364,OTTHUMP00000043365
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