IRS1 (phospho S639) polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human IRS1.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S639 of human IRS1.
Host
Rabbit
Theoretical MW (kDa)
180
Reactivity
Human, Mouse, Rabbit
Specificity
IRS1 (phospho S639) polyclonal antibody detects endogenous level of IRS1 only when phosphorylated at serine 639.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: 293 cells, Lane 2: serum treated 293 cells with IRS1 (phospho S639) polyclonal antibody (Cat# PAB29618) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by IRS1 (phospho S639) polyclonal antibody (Cat# PAB29618) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — IRS1
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Interactome
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Pathway
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Disease
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