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Last updated: 2017/8/13

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Nfkbie (phospho S22) polyclonal antibody

  • Catalog # : PAB29617
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of mouse Nfkbie.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S22 of mouse Nfkbie.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 40
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • Nfkbie (phospho S22) polyclonal antibody detects endogenous level of Nfkbie only when phosphorylated at serine 22.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: 293 cells, Lane 2: TNF treated 293 cells with Nfkbie (phospho S22) polyclonal antibody (Cat# PAB29617) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human breast cancer tissue by Nfkbie (phospho S22) polyclonal antibody (Cat# PAB29617) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • Nfkbie
  • Gene Alias:
  • IKBE
  • Gene Description:
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon
  • Other Designations:
  • -
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