Nfkbie (phospho S22) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: 293 cells, Lane 2: TNF treated 293 cells with Nfkbie (phospho S22) polyclonal antibody (Cat# PAB29617) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by Nfkbie (phospho S22) polyclonal antibody (Cat# PAB29617) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of mouse Nfkbie.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S22 of mouse Nfkbie.
Host
Rabbit
Theoretical MW (kDa)
40
Reactivity
Human, Mouse
Specificity
Nfkbie (phospho S22) polyclonal antibody detects endogenous level of Nfkbie only when phosphorylated at serine 22.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: 293 cells, Lane 2: TNF treated 293 cells with Nfkbie (phospho S22) polyclonal antibody (Cat# PAB29617) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by Nfkbie (phospho S22) polyclonal antibody (Cat# PAB29617) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — Nfkbie
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