HIST1H3A (phospho S10) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of Lane 1: SK-BR-3 cells, Lane 2: Insulin and EGF treated SK-BR-3 cells with HIST1H3A (phospho S10) polyclonal antibody (Cat# PAB29616) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by HIST1H3A (phospho S10) polyclonal antibody (Cat# PAB29616) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescent staining of methanol-fixed Hela cells using HIST1H3A (phospho S10) polyclonal antibody (Cat# PAB29616) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human HIST1H3A.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S10 of human HIST1H3A.
Host
Rabbit
Theoretical MW (kDa)
17
Reactivity
Human, Mouse, Rabbit
Specificity
HIST1H3A (phospho S10) polyclonal antibody detects endogenous level of HIST1H3A only when phosphorylated at serine 10.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: SK-BR-3 cells, Lane 2: Insulin and EGF treated SK-BR-3 cells with HIST1H3A (phospho S10) polyclonal antibody (Cat# PAB29616) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by HIST1H3A (phospho S10) polyclonal antibody (Cat# PAB29616) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.Immunofluorescence
Immunofluorescent staining of methanol-fixed Hela cells using HIST1H3A (phospho S10) polyclonal antibody (Cat# PAB29616) at 1:100-1:200 dilution. -
Gene Info — HIST1H3A
Entrez GeneID
8350Protein Accession#
P68431Gene Name
HIST1H3A
Gene Alias
H3/A, H3FA
Gene Description
histone cluster 1, H3a
Omim ID
602810Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member A|histone 1, H3a
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Interactome
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Pathway
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