Immunohistochemical staining of human breast cancer tissue by ABL1 (phospho Y412) polyclonal antibody (Cat# PAB29611) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
Immunofluorescent staining of methanol-fixed Hela cells using ABL1 (phospho Y412) polyclonal antibody (Cat# PAB29611) at 1:100-1:200 dilution.
The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq