MAPT (phospho T205) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Lane 1: mouse brain tissue lysate preincubated with blocking peptide, Lane 2: mouse brain tissue lysate without blocking peptide by MAPT (phospho T205) polyclonal antibody (Cat# PAB29607) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of rat hippocampal region tissue from a model with Alzheimer using MAPT (phospho T205) polyclonal antibody (Cat# PAB29607) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human MAPT.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T205 of human MAPT.
Host
Rabbit
Theoretical MW (kDa)
48, 62, 78
Reactivity
Human, Mouse, Rabbit
Specificity
MAPT (phospho T205) polyclonal antibody detects endogenous level of MAPT only when phosphorylated at threonine 205.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Lane 1: mouse brain tissue lysate preincubated with blocking peptide, Lane 2: mouse brain tissue lysate without blocking peptide by MAPT (phospho T205) polyclonal antibody (Cat# PAB29607) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of rat hippocampal region tissue from a model with Alzheimer using MAPT (phospho T205) polyclonal antibody (Cat# PAB29607) under 1:50-1:100 dilution. -
Gene Info — MAPT
Entrez GeneID
4137Protein Accession#
P10636Gene Name
MAPT
Gene Alias
DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU
Gene Description
microtubule-associated protein tau
Gene Ontology
HyperlinkGene Summary
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq
Other Designations
G protein beta1/gamma2 subunit-interacting factor 1|microtubule-associated protein tau, isoform 4
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Interactome
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Pathway
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Disease
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