Rps6ka1 (phospho T348) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cells, Lane 2: UV treated Hela cells with Rps6ka1 (phospho T348) polyclonal antibody (Cat# PAB29605) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by Rps6ka1 (phospho T348) polyclonal antibody (Cat# PAB29605) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescent staining of methanol-fixed Hela cells using Rps6ka1 (phospho T348) polyclonal antibody (Cat# PAB29605) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of mouse Rps6ka1.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T348 of mouse Rps6ka1.
Host
Rabbit
Theoretical MW (kDa)
90
Reactivity
Human, Mouse, Rabbit
Specificity
Rps6ka1 (phospho T348) polyclonal antibody detects endogenous level of Rps6ka1 only when phosphorylated at threonine 348.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cells, Lane 2: UV treated Hela cells with Rps6ka1 (phospho T348) polyclonal antibody (Cat# PAB29605) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by Rps6ka1 (phospho T348) polyclonal antibody (Cat# PAB29605) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.Immunofluorescence
Immunofluorescent staining of methanol-fixed Hela cells using Rps6ka1 (phospho T348) polyclonal antibody (Cat# PAB29605) at 1:100-1:200 dilution. -
Gene Info — Rps6ka1
Entrez GeneID
20111Protein Accession#
Q15418Gene Name
Rps6ka1
Gene Alias
Rsk1, p90rsk, rsk
Gene Description
ribosomal protein S6 kinase polypeptide 1
Gene Ontology
HyperlinkGene Summary
90kD
Other Designations
OTTMUSP00000010160|ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1
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