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IGF1R (phospho Y1165/Y1166) polyclonal antibody

  • Catalog # : PAB29602
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human IGF1R.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y1165/Y1166 of human IGF1R.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 200, 95
  • Reactivity:
  • Human, Mouse, Rabbit
  • Specificity:
  • IGF1R (phospho Y1165/Y1166) polyclonal antibody detects endogenous level of IGF1R only when phosphorylated at tyrosine 1165/tyrosine 1166.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunofluorescence (1:100-1:200)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: 293 cells, Lane 2: Insulin treated 293 cells with IGF1R (phospho Y1165/Y1166) polyclonal antibody (Cat# PAB29602) at 1:500-1:1000 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of methanol-fixed MCF-7 cells using IGF1R (phospho Y1165/Y1166) polyclonal antibody (Cat# PAB29602) at 1:100-1:200 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3480
  • Gene Name:
  • IGF1R
  • Gene Alias:
  • CD221,IGFIR,JTK13,MGC142170,MGC142172,MGC18216
  • Gene Description:
  • insulin-like growth factor 1 receptor
  • Gene Summary:
  • This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq
  • Other Designations:
  • soluble IGF1R variant 1,soluble IGF1R variant 2
  • Related Disease
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