GSK3A (phospho S21) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: 293 cells, Lane 2: serum treated 293 cells with GSK3A (phospho S21) polyclonal antibody (Cat# PAB29598) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by GSK3A (phospho S21) polyclonal antibody (Cat# PAB29598) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescent staining of methanol-fixed Hela cells shows positivity in cytoplasmic using GSK3A (phospho S21) polyclonal antibody (Cat# PAB29598) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human GSK3A.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S21 of human GSK3A.
Host
Rabbit
Theoretical MW (kDa)
51
Reactivity
Human, Mouse, Rabbit
Specificity
GSK3A (phospho S21) polyclonal antibody detects endogenous level of GSK3A only when phosphorylated at serine 21 and does not detect GSK3B when phosphorylated at serine 9.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: 293 cells, Lane 2: serum treated 293 cells with GSK3A (phospho S21) polyclonal antibody (Cat# PAB29598) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by GSK3A (phospho S21) polyclonal antibody (Cat# PAB29598) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.Immunofluorescence
Immunofluorescent staining of methanol-fixed Hela cells shows positivity in cytoplasmic using GSK3A (phospho S21) polyclonal antibody (Cat# PAB29598) at 1:100-1:200 dilution. -
Gene Info — GSK3A
Entrez GeneID
2931Protein Accession#
P49840Gene Name
GSK3A
Gene Alias
DKFZp686D0638
Gene Description
glycogen synthase kinase 3 alpha
Omim ID
606784Gene Ontology
HyperlinkGene Summary
Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM
Other Designations
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Interactome
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Disease
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