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GSK3A (phospho S21) polyclonal antibody

  • Catalog # : PAB29598
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human GSK3A.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S21 of human GSK3A.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 51
  • Reactivity:
  • Human, Mouse, Rabbit
  • Specificity:
  • GSK3A (phospho S21) polyclonal antibody detects endogenous level of GSK3A only when phosphorylated at serine 21 and does not detect GSK3B when phosphorylated at serine 9.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunofluorescence (1:100-1:200)
    Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: 293 cells, Lane 2: serum treated 293 cells with GSK3A (phospho S21) polyclonal antibody (Cat# PAB29598) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human breast cancer tissue by GSK3A (phospho S21) polyclonal antibody (Cat# PAB29598) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of methanol-fixed Hela cells shows positivity in cytoplasmic using GSK3A (phospho S21) polyclonal antibody (Cat# PAB29598) at 1:100-1:200 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2931
  • Gene Name:
  • GSK3A
  • Gene Alias:
  • DKFZp686D0638
  • Gene Description:
  • glycogen synthase kinase 3 alpha
  • Gene Summary:
  • Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM
  • Other Designations:
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