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ESPL1 (phospho S1126) polyclonal antibody

  • Catalog # : PAB29597
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human ESPL1.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S1126 of human ESPL1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 233
  • Reactivity:
  • Human
  • Specificity:
  • ESPL1 (phospho S1126) polyclonal antibody detects endogenous levels of human ESPL1 only when phosphorylated at serine 1126.
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Recommend Usage:
  • Immunofluorescence (1:20-1:100)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of U-2 OS cells with ESPL1 (phospho S1126) polyclonal antibody (Cat # PAB29597) at 1:20-1:100 dilution. Blue: DAPI for nuclear staining.
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 9700
  • Gene Name:
  • ESPL1
  • Gene Alias:
  • ESP1,FLJ46492,KIAA0165,SEPARASE,SEPARIN
  • Gene Description:
  • extra spindle pole bodies homolog 1 (S. cerevisiae)
  • Gene Summary:
  • Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 604359) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM
  • Other Designations:
  • extra spindle poles like 1,separin, separase
  • Gene Pathway
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