Western blot analysis of Lane 1: Untreated 293 cells, Lane 2: Insulin treated 293 cells, Lane 3: Untreated HeLa cells, Lane 4: UV treated HeLa cells with RB1 (phospho S811) polyclonal antibody (Cat # PAB29596) at 1:500-1:2000 dilution.
Immunofluorescent staining of U-2 OS cells with RB1 (phospho S811) polyclonal antibody (Cat # PAB29596) at 1:50-1:200 dilution. Blue: DAPI for nuclear staining.
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
OTTHUMP00000018397,retinoblastoma suspectibility protein,retinoblastoma-associated protein