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DNMT1 (phospho Y399) polyclonal antibody

  • Catalog # : PAB29593
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human DNMT1.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y399 of human DNMT1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 200
  • Reactivity:
  • Human
  • Specificity:
  • DNMT1 (phospho Y399) polyclonal antibody detects endogenous levels of human DNMT1 only when phosphorylated at tyrosine 399.
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Recommend Usage:
  • Immunofluorescence (1:50-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of MCF-7 cells with DNMT1 (phospho Y399) polyclonal antibody (Cat # PAB29593) at 1:50-1:200 dilution. Blue: DAPI for nuclear staining.
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1786
  • Gene Name:
  • DNMT1
  • Gene Alias:
  • AIM,CXXC9,DNMT,FLJ16293,MCMT,MGC104992
  • Gene Description:
  • DNA (cytosine-5-)-methyltransferase 1
  • Gene Summary:
  • DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • CXXC finger protein 9,DNA methyltransferase 1
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