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Last updated: 2016/12/11

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CHEK1 (phospho S317) polyclonal antibody

  • Catalog # : PAB29591
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human CHEK1.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S317 of human CHEK1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 54
  • Reactivity:
  • Human
  • Specificity:
  • CHEK1 (phospho S317) polyclonal antibody detects endogenous levels of human CHEK1 only when phosphorylated at serine 317.
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Recommend Usage:
  • Immunofluorescence (1:50-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Untreated MCF-7 cells, Lane 2: Peptide treated MCF-7 cells with CHEK1 (phospho S317) polyclonal antibody (Cat # PAB29591) at 1:500-1:2000 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of MCF-7 cells (A1 and A2) and U-2 OS cells (B1 and B2) with CHEK1 (phospho S317) polyclonal antibody (Cat # PAB29591) at 1:50-1:200 dilution. Blue: DAPI for nuclear staining.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1111
  • Gene Name:
  • CHEK1
  • Gene Alias:
  • CHK1
  • Gene Description:
  • CHK1 checkpoint homolog (S. pombe)
  • Other Designations:
  • CHK1 checkpoint homolog,Checkpoint, S. pombe, homolog of, 1
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