CHEK1 (phospho S317) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated MCF-7 cells, Lane 2: Peptide treated MCF-7 cells with CHEK1 (phospho S317) polyclonal antibody (Cat # PAB29591) at 1:500-1:2000 dilution.
Immunofluorescence
Immunofluorescent staining of MCF-7 cells (A1 and A2) and U-2 OS cells (B1 and B2) with CHEK1 (phospho S317) polyclonal antibody (Cat # PAB29591) at 1:50-1:200 dilution. Blue: DAPI for nuclear staining.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human CHEK1.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S317 of human CHEK1.
Host
Rabbit
Theoretical MW (kDa)
54
Reactivity
Human
Specificity
CHEK1 (phospho S317) polyclonal antibody detects endogenous levels of human CHEK1 only when phosphorylated at serine 317.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated MCF-7 cells, Lane 2: Peptide treated MCF-7 cells with CHEK1 (phospho S317) polyclonal antibody (Cat # PAB29591) at 1:500-1:2000 dilution.Immunofluorescence
Immunofluorescent staining of MCF-7 cells (A1 and A2) and U-2 OS cells (B1 and B2) with CHEK1 (phospho S317) polyclonal antibody (Cat # PAB29591) at 1:50-1:200 dilution. Blue: DAPI for nuclear staining. -
Gene Info — CHEK1
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Interactome
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Pathway
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Disease
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