Western blot analysis of Lane 1: Untreated MCF-7 cells, Lane 2: Peptide treated MCF-7 cells with CHEK1 (phospho S317) polyclonal antibody (Cat # PAB29591) at 1:500-1:2000 dilution.
Immunofluorescence
Immunofluorescent staining of MCF-7 cells (A1 and A2) and U-2 OS cells (B1 and B2) with CHEK1 (phospho S317) polyclonal antibody (Cat # PAB29591) at 1:50-1:200 dilution. Blue: DAPI for nuclear staining.