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WASF1 (phospho Y125) polyclonal antibody

  • Catalog # : PAB29590
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human WASF1.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y125 of human WASF1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 70
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • WASF1 (phospho Y125) polyclonal antibody detects endogenous levels of human WASF1 only when phosphorylated at tyrosine 125.
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Insulin (0.01 U/mL, 15 mins) treated NIH/3T3 cells, Lane 2: Synthesized peptide treated NIH/3T3 cells with WASF1 (phospho Y125) polyclonal antibody (Cat # PAB29590) at 1:500-1:3000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon carcinoma tissue with WASF1 (phospho Y125) polyclonal antibody (Cat # PAB29590) under 1:50-1:100 dilution (A) or treated with synthesized peptide (B).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8936
  • Gene Name:
  • WASF1
  • Gene Alias:
  • FLJ31482,KIAA0269,SCAR1,WAVE,WAVE1
  • Gene Description:
  • WAS protein family, member 1
  • Gene Summary:
  • The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000016990,Wiskott-Aldrich syndrome protein family member 1,homology of dictyostelium scar 1,verprolin homology domain-containing protein 1
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