UBTF (phospho S484) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: calyculinA (50 ng/mL, 30 mins) treated HeLa cells, Lane 2: Synthesized peptide treated HeLa cells with UBTF (phospho S484) polyclonal antibody (Cat # PAB29589) at 1:500-1:3000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast carcinoma tissue with UBTF (phospho S484) polyclonal antibody (Cat # PAB29589) under 1:50-1:100 dilution (A) or treated with synthesized peptide (B).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human UBTF.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S484 of human UBTF.
Host
Rabbit
Theoretical MW (kDa)
85
Reactivity
Human, Mouse, Rat
Specificity
UBTF (phospho S484) polyclonal antibody detects endogenous levels of human UBTF only when phosphorylated at serine 484.
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: calyculinA (50 ng/mL, 30 mins) treated HeLa cells, Lane 2: Synthesized peptide treated HeLa cells with UBTF (phospho S484) polyclonal antibody (Cat # PAB29589) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast carcinoma tissue with UBTF (phospho S484) polyclonal antibody (Cat # PAB29589) under 1:50-1:100 dilution (A) or treated with synthesized peptide (B). -
Gene Info — UBTF
Entrez GeneID
7343Protein Accession#
P17480Gene Name
UBTF
Gene Alias
NOR-90, UBF
Gene Description
upstream binding transcription factor, RNA polymerase I
Omim ID
600673Gene Ontology
HyperlinkGene Summary
Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM
Other Designations
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