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UBTF (phospho S484) polyclonal antibody

  • Catalog # : PAB29589
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human UBTF.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S484 of human UBTF.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 85
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • UBTF (phospho S484) polyclonal antibody detects endogenous levels of human UBTF only when phosphorylated at serine 484.
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: calyculinA (50 ng/mL, 30 mins) treated HeLa cells, Lane 2: Synthesized peptide treated HeLa cells with UBTF (phospho S484) polyclonal antibody (Cat # PAB29589) at 1:500-1:3000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast carcinoma tissue with UBTF (phospho S484) polyclonal antibody (Cat # PAB29589) under 1:50-1:100 dilution (A) or treated with synthesized peptide (B).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7343
  • Gene Name:
  • UBTF
  • Gene Alias:
  • NOR-90,UBF
  • Gene Description:
  • upstream binding transcription factor, RNA polymerase I
  • Gene Summary:
  • Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM
  • Other Designations:
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