Western blot analysis of Lane 1: calyculinA (50 ng/mL, 30 mins) treated HeLa cells, Lane 2: Synthesized peptide treated HeLa cells with UBTF (phospho S484) polyclonal antibody (Cat # PAB29589) at 1:500-1:3000 dilution.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast carcinoma tissue with UBTF (phospho S484) polyclonal antibody (Cat # PAB29589) under 1:50-1:100 dilution (A) or treated with synthesized peptide (B).
Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM