SRF (phospho S77) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: PMA (125 ng/mL, 30 mins) treated Jurkat cells, Lane 2: Synthesized peptide treated Jurkat cells with SRF (phospho S77) polyclonal antibody (Cat # PAB29587) at 1:500-1:3000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain tissue with SRF (phospho S77) polyclonal antibody (Cat # PAB29587) under 1:50-1:100 dilution (A) or treated with synthesized peptide (B).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human SRF.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S77 of human SRF.
Host
Rabbit
Theoretical MW (kDa)
52
Reactivity
Human, Mouse
Specificity
SRF (phospho S77) polyclonal antibody detects endogenous levels of human SRF only when phosphorylated at serine 77.
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: PMA (125 ng/mL, 30 mins) treated Jurkat cells, Lane 2: Synthesized peptide treated Jurkat cells with SRF (phospho S77) polyclonal antibody (Cat # PAB29587) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain tissue with SRF (phospho S77) polyclonal antibody (Cat # PAB29587) under 1:50-1:100 dilution (A) or treated with synthesized peptide (B). -
Gene Info — SRF
Entrez GeneID
6722Protein Accession#
P11831Gene Name
SRF
Gene Alias
MCM1
Gene Description
serum response factor (c-fos serum response element-binding transcription factor)
Omim ID
600589Gene Ontology
HyperlinkGene Summary
This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). [provided by RefSeq
Other Designations
OTTHUMP00000039820
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Interactome
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Pathway
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Disease
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