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MAP1LC3A polyclonal antibody

  • Catalog # : PAB29558
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial synthetic protein of human MAP1LC3A.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 1-30 at N-terminus of human MAP1LC3A.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 14.3
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Isotype:
  • Ig
  • Recommend Usage:
  • Immunofluorescence (1:200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of Lane 1: Rat brain tissue lysates, Lane 2: Rat brain tissue lysates treated with blocking peptide with MAP1LC3A polyclonal antibody (Cat # PAB29558) at 1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain tissue with MAP1LC3A polyclonal antibody (Cat # PAB29558) at 1:50-100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of Chloroquine (50 uM, 16h) treated U251 cells with MAP1LC3A polyclonal antibody (Cat # PAB29558) shows positivity in cytoplasmic autophagic vacuoles at 1:200 dilution.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • MAP1LC3A
  • Gene Alias:
  • LC3,LC3A,MAP1ALC3,MAP1BLC3
  • Gene Description:
  • microtubule-associated protein 1 light chain 3 alpha
  • Gene Summary:
  • MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • MAP1 light chain 3-like protein 1,MAP1A/1B light chain 3 A,MAP1A/MAP1B LC3 A,OTTHUMP00000030696,OTTHUMP00000030697,OTTHUMP00000030698,microtubule-associated proteins 1A/1B light chain 3
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